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Photomicrographs of the post-thawed sperm stained with MMP <t>(mitochondrial</t> membrane potentials) using JC-1 fluorescent probes and DNA using acridine orange (AO) staining. Images (A,B) were obtained under a phase-contrast microscope. In image (A) , the blue arrow indicated intact DNA and sperm with intensively bright green fluorescence of the DNA, which indicated by an intact DNA integrity. The white arrow indicated damaged DNA and sperm with red fluorescence, which indicated DNA damage. In image (B) , the blue arrow indicated sperm with a high level of MMP (high orange fluorescence level), and the white arrow indicated sperm with a low MMP level (low orange fluorescence level).
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Photomicrographs of the post-thawed sperm stained with MMP (mitochondrial membrane potentials) using JC-1 fluorescent probes and DNA using acridine orange (AO) staining. Images (A,B) were obtained under a phase-contrast microscope. In image (A) , the blue arrow indicated intact DNA and sperm with intensively bright green fluorescence of the DNA, which indicated by an intact DNA integrity. The white arrow indicated damaged DNA and sperm with red fluorescence, which indicated DNA damage. In image (B) , the blue arrow indicated sperm with a high level of MMP (high orange fluorescence level), and the white arrow indicated sperm with a low MMP level (low orange fluorescence level).

Journal: Frontiers in Veterinary Science

Article Title: Effects of hesperidin and ascorbic acid combination on boar sperm quality during cryopreservation

doi: 10.3389/fvets.2025.1573983

Figure Lengend Snippet: Photomicrographs of the post-thawed sperm stained with MMP (mitochondrial membrane potentials) using JC-1 fluorescent probes and DNA using acridine orange (AO) staining. Images (A,B) were obtained under a phase-contrast microscope. In image (A) , the blue arrow indicated intact DNA and sperm with intensively bright green fluorescence of the DNA, which indicated by an intact DNA integrity. The white arrow indicated damaged DNA and sperm with red fluorescence, which indicated DNA damage. In image (B) , the blue arrow indicated sperm with a high level of MMP (high orange fluorescence level), and the white arrow indicated sperm with a low MMP level (low orange fluorescence level).

Article Snippet: Sperm mitochondrial activity was determined using a Mitochondrial Membrane Potential Detection Kit (C2003S, Beyotime Institute of Biotechnology, China), as described by Dong et al. ( ).

Techniques: Staining, Membrane, Microscopy, Fluorescence

Effects of Hsd and AsA supplementation on the physiological characteristics and ATP content of frozen-thawed boar sperm in control and at 10 min after thawing. (a) Membrane integrity, (b) acrosome integrity, (c) mitochondrial membrane potentials, (d) DNA integrity, (e) ATP content.

Journal: Frontiers in Veterinary Science

Article Title: Effects of hesperidin and ascorbic acid combination on boar sperm quality during cryopreservation

doi: 10.3389/fvets.2025.1573983

Figure Lengend Snippet: Effects of Hsd and AsA supplementation on the physiological characteristics and ATP content of frozen-thawed boar sperm in control and at 10 min after thawing. (a) Membrane integrity, (b) acrosome integrity, (c) mitochondrial membrane potentials, (d) DNA integrity, (e) ATP content.

Article Snippet: Sperm mitochondrial activity was determined using a Mitochondrial Membrane Potential Detection Kit (C2003S, Beyotime Institute of Biotechnology, China), as described by Dong et al. ( ).

Techniques: Control, Membrane